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For his Grands Voyages, De Bry engraved 42 plates based Le Moyne's original sketches made during the French Huguenot voyage to the Florida Peninsula. De Bry's renderings of Florida and its inhabitants are today the earliest known printed European images of Native Americans in present-day Florida, known as the Timucua Indians.
The images attempt to convey a number of messages about the land and its peoples. For example, some of the plates have been suggested to represent the ability of the Timucua to obey authority and that they are less sophisticated than the Europeans. This was argued to make them ideal candidates for French Huegonot colonization, to be used in conflicts against Catholic Spain.
The accuracy of De Bry's depictions have sometimes been called into question. Some of the engravings do not quite match what became known about the Timucua by later French explorers, and some engravings possess out-of-place features, such as the appearance of a Pacific nautilus rather than a Florida whelk shell. However, it is believed that the core of the imagery is correct. For example, the depiction of Timucuan body art, otherwise unknown to Europeans at the time, suggest that De Bry's depictions were grounded in reality.